種屬來源：人

組織來源：神經膠質

特征：神經膠質細胞瘤

細胞形態：成纖維細胞樣

生長特性：貼壁生長

培養體系：DMEM-H+10%FBS+1%P/S

u251細胞株-人膠質瘤細胞 配套培養基貨號： TCH-G366

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎培養基+30%FBS+10%DMSO，液氮儲存

質量檢測：細菌、真菌、支原體檢測均為陰性

u251細胞株-人膠質瘤細胞 分離于膠質母細胞瘤組織。 

"Dutil J., Chen Z., Monteiro A.N., Teer J.K., Eschrich S.A.

An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.

Cancer Res. 79:1263-1273(2019)

Li J., Zhao W., Akbani R., Liu W., Ju Z., Ling S., Vellano C.P., Roebuck P., Yu Q., Eterovic A.K., Byers L.A., Davies M.A., Deng W., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y., Mills G.B., Liang H.

Characterization of human cancer cell lines by reverse-phase protein arrays.

Cancer Cell 31:225-239(2017)

Zampella J.G., Rodic N., Yang W.R., Huang C.R.L., Welch J., Gnanakkan V.P., Cornish T.C., Boeke J.D., Burns K.H.

A map of DNA insertions in the NCI-60 human cancer cell panel.

Mob. DNA 7:20-20(2016)

Allen M., Bjerke M., Edlund H., Nelander S., Westermark B.

Origin of the U87MG glioma cell line: good news and bad news.

Sci. Transl. Med. 8:354re3-354re3(2016)

Mestdagh P., Lefever S., Volders P.-J., Derveaux S., Hellemans J., Vandesompele J.

Long non-coding RNA expression profiling in the NCI60 cancer cell line panel using high-throughput RT-qPCR.

Sci. Data 3:160052-160052(2016)"