c2c12細(xì)胞-小鼠成肌細(xì)胞

細(xì)胞名稱： c2c12細(xì)胞-小鼠成肌細(xì)胞

細(xì)胞別稱： C2c12; C2-C12; C12

貨號(hào)：TCM-C720

規(guī)格： 1X10^6/T25細(xì)胞培養(yǎng)瓶

價(jià)格：1500元 (干冰運(yùn)輸需另加200元干冰費(fèi) )  

產(chǎn)品貨號(hào)：TCM-C720

種屬來(lái)源：小鼠

組織來(lái)源：肌肉

細(xì)胞形態(tài)：成肌細(xì)胞樣

生長(zhǎng)特性：貼壁生長(zhǎng)

培養(yǎng)體系：DMEM-H+10%FBS+1%Glutamax+1% Sodium Pyruvate+1%P/S

c2c12細(xì)胞-小鼠成肌細(xì)胞  配套培養(yǎng)基貨號(hào)：TCM-G720

傳代比例：1:3-1:6，每2-3天換液一次

傳代周期：48-72 h

培養(yǎng)條件：氣相：95%空氣+5%CO2，溫度：37℃

凍存條件：60%基礎(chǔ)培養(yǎng)基+30%FBS+10%DMSO，液氮儲(chǔ)存

質(zhì)量檢測(cè)：細(xì)菌、真菌、支原體檢測(cè)均為陰性

 c2c12細(xì)胞-小鼠成肌細(xì)胞  細(xì)胞株是YaffeD,SaxelO建立的小鼠成肌細(xì)胞系的亞株。該細(xì)胞分化較快，可形成能收縮的微管，產(chǎn)生特異的肌肉蛋白。在骨形態(tài)形成蛋白（BMP-2）的作用下，  c2c12細(xì)胞可由成肌細(xì)胞分化為成骨細(xì)胞。

參考文獻(xiàn)：

"Qing Y, et al. Inhibitory effects of iron on bone morphogenetic protein 2-induced osteoblastogenesis. J. Bone Miner. Res. 26(6): 1188-1196, 2011. PubMed:

Chow YH, et al. Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2. J. Virol. 71: 169-178, 1997. PubMed: 8985336

Hsu DK, et al. Identification of a murine TEF-1-related gene expressed after mitogenic stimulation of quiescent fibroblasts and during myogenic differentiation. J. Biol. Chem. 271: 13786-13795, 1996. PubMed: 8662936

Kessler PD, et al. Gene delivery to skeletal muscle results in sustanined expression and systemic delivery of a therapeutic protein. Proc. Natl. Acad. Sci. USA 93: 14082-14087, 1996. PubMed: 8943064

Katagiri T, et al. Bone morphogenetic protein-2 converts the differentiation pathway of C2C12 myoblasts into the osteoblast lineage [published erratum appears in J Cell Biol 1995 Feb;128(4):following 713]. J. Cell Biol. 127: 1755-1766, 1994. PubMed: 7798324

Blau HM, et al. Plasticity of the differentiated state. Science 230: 758-766, 1985. PubMed: 2414846

Yaffe D, Saxel O. Serial passaging and differentiation of myogenic cells isolated from dystrophic mouse muscle. Nature 270: 725-727, 1977. PubMed: 563524"