六月，驕陽似火，暑氣留戀。大街上，涌動的人流，五顏六色的衣妝，匯成橫亙天地間的七色彩虹，描繪著生活美不勝收的詩情畫意。

我們進入六月，一陣陣熱浪撲面而來，整個世界都被烘烤得滾燙滾燙。也開始不再靜下心來，煩躁，一觸即發，但如果靜心下來就會平淡如水，了無波痕。

而就是這樣的六月我們的南京林業大學的老師使用上海滬鼎生物自主品牌ELISA試劑盒在《Overexpression of the LcCUC2-like》期刊上發表的SCI文獻—— gene in Arabidopsis thaliana alters thecotyledon morphology and increasesrosette leaf number順利通過，對生物科研方面有了新的的貢獻。

精彩的文獻不僅可以讓我們更好的學習，還可以起到*作用，希望對正在做實驗和寫論文的科研小伙伴們有所幫助，通過文獻我們可以更好的了解到產品，如果您也使用滬鼎品牌的ELISA試劑盒發表了SCI文獻，歡迎您積極的參與我們的“fa論文贏獎學金"活動，小編等著你們的投稿哦，下一個高額領獎金的說不定就是你！

【文獻標題】Overexpression of the LcCUC2-likegene in Arabidopsis thaliana alters thecotyledon morphology and increasesrosette leaf number

【DOI】Wen et al. (2022), PeerJ, DOI 10.7717/peerj.12615

【作者單位】南京林業大學 《Nanjing Forestry University》

【作者】Shaoying Wen1, Jiayu Li1

【產品文獻支持】Auxin content measurement

Basal rosette leaves samples of 0.6 g were harvested from 3-week-old WT and LcCUC2L

transgenic A. thaliana plants and immediately frozen in liquid nitrogen. Determination

of indole-3-acetic acid (IAA) in A. thaliana leaves was performed by ELISA (Huding,

Shanghai, China). Three replicates were used for each sample.

影響因子(IF)= ：2.98

【出版期刊】：《PlantPhysiology and Biochemistry》

【產品原文引用】

Auxin content measurement

Basal rosette leaves samples of 0.6 g were harvested from 3-week-old WT and LcCUC2L

transgenic A. thaliana plants and immediately frozen in liquid nitrogen. Determination

of indole-3-acetic acid (IAA) in A. thaliana leaves was performed by ELISA (Huding,

Shanghai, China). Three replicates were used for each sample.

Histochemical staining to detect GUS activity

The β-Galactosidase Reporter Gene Staining Kit (Leagene) was used for GUS histochemical

staining in ProLcCUC2L transgenic and WT A. thaliana plants. In this study, seven-day-old

seedlings and fourteen-day-old seedlings were processed. Moreover, leaves at different

stages of development (10, 20 and 30 days of age) from plants planted in soil were

subjected to GUS staining. All materials were treated with GUS staining solution and

incubated overnight at 37 ?C, followed by bleaching with 75% (v/v) ethanol.

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