當前位置:上海莼試生物技術有限公司>>分子生物學>>細胞培養及檢測>> 吖啶橙(AO)-EB雙染色試劑盒使用說明書
吖啶橙(AO)-EB雙染色試劑盒使用說明書*,*,庫存現貨,無效果,免費退款。公司現貨銷售,量大折扣,咨詢。詳細信息如下。
吖啶橙(AO)-EB雙染色試劑盒使用說明書產品簡介:
吖啶橙能透過胞膜完整的細胞,嵌入細胞核DNA,與雙鏈DNA結合后發出綠色熒光,溴化乙錠(EB)僅能透過胞膜受損的細胞,嵌入核DNA,發橘紅色熒光。凋亡的細胞呈現為染色增強,熒光更為明亮,均勻*的圓狀或固縮狀、團塊狀結構。非凋亡細胞核呈現熒光深淺不一的結構樣特征。二者很容易判別。在熒光顯微鏡下觀察,可見四種細胞形態:活細胞(VN),核染色質著綠色并呈正常結構;早期凋亡細胞(VA),核染色質著綠色呈固縮狀或圓珠狀;晚期凋亡細胞(NVA),核染色質為橘紅色并呈固縮狀或圓珠狀;非凋亡的死亡細胞(NVN),核染色質著橘紅色并呈正常結構。本產品AO、EB溶液濃度分別為100ug/ml,所含穩定劑不影響實驗效果。
儲存條件:
4℃避光密封保存,有效期一年。
吖啶橙(AO)-EB雙染色試劑盒使用說明書分子生物學領域:
涉及核酸純化、常用分子生物學試劑、PCR等相關試劑盒。
分子生物學試劑:擁有的價格優勢,品質可信賴,市場占比龐大。
產品名稱 | 英文名稱 | 包裝 |
吖啶橙(AO)-EB雙染色試劑盒使用說明書 | AO-EB double staining kit | 可根據客戶要求訂制 |
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CFN90454 http://www.chemfaces。。com/natural/Solanesol-CFN90454.html C45H74O 631.07 13190-97-1 Solanesol Miscellaneous >=98% 20mg
CFN90456 http://www.chemfaces。。com/natural/Soyasaponin-Bb-CFN90456.html C48H78O18 943.12 51330-27-9 Soyasaponin Bb Triterpenoids >=97% 20mg
CFN90461 http://www.chemfaces。。com/natural/Thermopsine-CFN90461.html C15H20N2O 244.33 486-90-8 Thermopsine Alkaloids >=98% 10mg
CFN90462 http://www.chemfaces。。com/natural/Topotecan-hydrochloride-CFN90462.html C23H24ClN3O5 457.9 119413-54-6 Topotecan hydrochloride Alkaloids >=98% 20mg
CFN90463 http://www.chemfaces。。com/natural/Vanillin-CFN90463.html C8H8O3 152.14 121-33-5 Vanillin Phenols >=98% 100mg
CFN90467 http://www.chemfaces。。com/natural/Vinpocetine-CFN90467.html C22H26N2O2 350.45 42971-09-5 Vinpocetine Alkaloids >=98% 20mg
CFN90475 http://www.chemfaces。。com/natural/Hupehenine-CFN90475.html C27H45NO2 415.65 98243-57-3 Hupehenine Alkaloids >=98% 10mg
IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蠟切片需做抗原修復)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
產品介紹 ARHGEF7 is a p21-activated protein kinase (Pak)-interacting exchange factor that has been identified as a putative guanine nucleotide exchange factor (GEF) for Rac/Cdc42. It is expressed ubiquitously in all the tissue examined and other isoforms are expressed mainly in the central nervous system. Regulated 吖啶橙(AO)-EB雙染色試劑盒使用說明書phosphorylation of ARHGEF7 is necessary to maintain the balance between normal signalling by EGFR and Src versus aberrant growth and transformation. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene, but some of their full-length sequences have not been
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